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Literature summary extracted from

  • Bally, I.; Ancelet, S.; Moriscot, C.; Gonnet, F.; Mantovani, A.; Daniel, R.; Schoehn, G.; Arlaud, G.J.; Thielens, N.M.
    Expression of recombinant human complement C1q allows identification of the C1r/C1s-binding sites (2013), Proc. Natl. Acad. Sci. USA, 110, 8650-8655.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.41 development and evaluation of a method for expression of recombinant full-length human C-terminally tagged C1q involving stable transfection of HEK 293-F mammalian cells Homo sapiens
3.4.21.42 development and evaluation of a method for expression of recombinant full-length human C-terminally tagged C1q involving stable transfection of HEK 293-F mammalian cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.41 extracellular
-
 Homo sapiens
-
-
3.4.21.42 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.41 complement C1q zymogen + H2O Homo sapiens
-
 active complement C1q + ?
-
 ?
3.4.21.42 complement C1q zymogen + H2O Homo sapiens
-
 active complement C1q + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.41 Homo sapiens P00736
-
-
3.4.21.42 Homo sapiens P09871
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.41 blood serum
-
 Homo sapiens
-
3.4.21.42 blood serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.41 complement C1q zymogen + H2O
-
 Homo sapiens active complement C1q + ?
-
 ?
3.4.21.41 complement C1q zymogen + H2O no or reduced activity with substrate mutants K59A, K61A, K58A, and K58A/K59A/K61A Homo sapiens active complement C1q + ?
-
 ?
3.4.21.42 complement C1q zymogen + H2O
-
 Homo sapiens active complement C1q + ?
-
 ?
3.4.21.42 complement C1q zymogen + H2O no or reduced activity with substrate mutants K59A, K61A, K58A, and K58A/K59A/K61A Homo sapiens active complement C1q + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.41 proteases C1r
-
 Homo sapiens
3.4.21.42 protease C1s
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.41 37
-
 assay at Homo sapiens
3.4.21.42 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.41 7.4
-
 assay at Homo sapiens
3.4.21.42 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.41 metabolism hexameric complement C1q is a versatile recognition protein that senses a wide variety of immune and nonimmune ligands, including pathogens and altered self components, and triggers the classical complement pathway through activation of its associated proteases C1r and C1s, EC 3.4.21.42. Residues LysB61 and LysC58 each play a key role in the interaction with C1s-C1r-C1r-C1s, with LysA59 being involved to a lesser degree Homo sapiens
3.4.21.42 metabolism hexameric complement C1q is a versatile recognition protein that senses a wide variety of immune and nonimmune ligands, including pathogens and altered self components, and triggers the classical complement pathway through activation of its associated proteases C1r, EC 3.4.21.41, and C1s. Residues LysB61 and LysC58 each play a key role in the interaction with C1s-C1r-C1r-C1s, with LysA59 being involved to a lesser degree Homo sapiens